INVESTIGADORES
MALDONADO GALDEANO Maria Carolina
congresos y reuniones científicas
Título:
Sub-cellular fractions of probiotic bacteria administered by oral route induce peritoneal macrophages activation
Autor/es:
LEMME DUMIT, JOSÉ MARÍA; MALDONADO GALDEANO, CAROLINA; PERDIGÓN, GABRIELA.
Lugar:
Mar del Plata
Reunión:
Congreso; LIX reunion Cientifica anual de la Sociedad Argentina de Investigación Clínica (SAIC). LXII Reunión Anual de la Sociedad de Inmunología (SAI); 2014
Institución organizadora:
Sociedad Argentina de Inmunologia
Resumen:
The ability to affect the host immune response is a feature of importance in the application of the probiotic bacteria strains. The identifying and characterizing of bacterial components that stimulate the immune system is crucial for the elucidation of novel structure as oral adjuvant. Aim: To evaluate the impact of sub-cellular components: exopolysaccharide (EPS), intracellular components (IC) and wall cellular (WC) of probiotic bacteria by in vitro and ex vivo assays, on the activity of peritoneal macrophages (MQP) to be the sentinels of immune innate response. We compared with a commensal bacterium. Different fractions were obtained from two probiotic bacteria: Lactobacillus casei CRL 431 (Lc), Lactobacillus paracasei CNCMI-1518 (Lp) and a commensal bacteria: Lactobacillus acidophilus CRL 730 (La). In vitro, the production of IL-6 was determined by ELISA in the supernatant of MQP stimulated with the sub-cellular fractions. Ex vivo, BALB/c mice received by oral route: EPS, IC and WC of Lc and La for 7 days, and for Lp 5 days. Phagocytic and antimicrobial activities of MQP were determined. Results: In vitro, IL-6 levels increases significantly (P≤0.05) when the cells were stimulated with IC and WC for probiotics Lc and Lp, and with EPS for La. In vivo, phagoytic and antimicrobial activities of MQP were enhanced for the WC (P≤0.05) of both probiotic strains. Phagocytic activity increases also with IC from La. Conclusion: We showed that the main immunomodulatory effect of probiotic bacteria (Lc, Lp) are in the wall cellular and exopolysaccharide for the commensal bacteria (La). The characterization of probiotic components able to act as oral adjuvant is an approach to elucidate the molecules involved in such effect.