Systemic and innate immunity in stressed mice during probiotics consumptions

PALOMAR, MARTÍN; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA

Córdoba

Congreso; LVIII Reunión Científica Anual Sociedad Argentina de Investigación Clínica (SAIC)-LX Reunión Anual Sociedad Argentina de Inmunología (SAI).; 2013

The gastrointestinal tract and the immune system are particularly responsive to different stressor factors. In previous work, stressed mice showed changes in the intestinal microbiota composition and decrease in the number of IgA-producing cells. The aim of this work was to determine whether changes in gut-level affect the systemic Immunity, and how the probiotics administration can reverse the effects induced by stress. BALB / c adults mice were subjected over 11 days to a stress protocol consisting of food deprivation during 12h/day (8:00 pm-8: 00am) and diurnal mobilization for 3h. Mice were divided into 9 groups: G1:normal control (C); G2:normal control+OVA; G3:Lactobacillus casei (Lc)+OVA; G4:probiotic fermented milk (PFM)+OVA; G5:Stressed mice (S)+OVA; G6: S+Lc+OVA+Lc; G7:S+PFM+OVA+PFM; G8: S+Lc+OVA+water and G9: S+PFM+OVA+water. Mice were immunized with ovalbumin. Samples were taken at 10 days post-sensitization: serum for specific-IgG determination; small intestine for morphometry studies, peritoneal and spleen macrophages for phagocytic capacity determination. The stress induced a significant shortening of the length of the small intestine and diminished the peritoneal and spleen macrophages activity (P≤0,05). The systemic immunity was evaluated by specific-OVA-IgG determination in serum. villi. Probiotics administration restored the length of the intestinal microvilli and induced increases in the IgG levels in serum. The Lc administration enhanced the phagocytic activity of the peritoneal macrophages to values near to the G1 (P≤0,05). In the spleen the major effect was obtained with the PFM administration. Probiotic and PFM administration recovers he intestinal physiology and improves systemic immune response in stressed mice. The phagocytic activity of peritoneal and spleen macrophages partially recovered, without reaching normal control values. So the consumption of probiotics could be useful to improve the immune state in stressed host