INVESTIGADORES
MALDONADO GALDEANO Maria Carolina
congresos y reuniones científicas
Título:
Effect of a probiotic strain and a probiotic fermented milk on the gut immune response in a stress model in mouse
Autor/es:
PALOMAR, MARTÍN; CARMUEGA, ESTEBAN; WEILL, RICARDO; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA
Lugar:
Vancouver
Reunión:
Congreso; 16th International Congress of Mucosal Immunology.; 2013
Institución organizadora:
Society of Mucosal Immunology
Resumen:
Stress negatively influences the immune system and gastrointestinal physiology. In a mouse stress model, we evaluated the effect of the probiotic strains Lactobacillus casei (Lc) CRL-431 and a probiotic fermented milk (PFM) containing Lc DN-114001 in immune recovery such us the number of IgA+ cells in small intestine and cytokines levels released from in intestinal epithelial cells (IEC), spleen (SM) and peritoneal macrophages (PM) supernatant culture. Mice were divided into 4 groups: normal-control (NC), stress-control (SC) and stressed mice fed with Lc (S+Lc) or with PFM (S+PFM). INF-γ levels decreased in IEC supernatant in S+Lc group compared to the NC. The IL-6 levels increased significantly in stressed mice compared to NC, while probiotic or PFM administration maintained normal IL-6 levels. The PFM administration induced an important IL-10 production in the supernatant of SM culture with lower levels of IL-6 compared to the control groups, while the opposite effect was observed in the culture of PM. Probiotic administration restored the number of IgA+ cells in stressed mice. Stress induces an inflammatory state in the intestine with increases of IL-6 and IFN-γ and decreased IgA+ cells. Probiotic or PFM administration normalized IgA levels and regulates the intestinal immune response with IL-10 increases.