Regulation exert by a probiotic fermented milk in a respiratory experimental model of Allergy

CAROLINA, MALDONADO GALDEANO; VELEZ EVA; BIBAS BONET, EUGENIA; MESON OSCAR; CARMUEGA, ESTEBAN; WEILL, RICARDO; PERDIGÓN, GABRIELA

Paris

Congreso; 15th International Congress of Mucosal Immunology; 2011

Society of Mucosal Immunology

Mechanisms involved in the beneficial effects of functional food are not well known. We studied the immune mechanisms mediated by probiotic fermented milk (PFM) in an allergy mice model. Experimental groups: normal-control (NC), Basal (B-5days-PFM); OVA-Sensitization-control (SC), Previous (P)(5d-PFM+OVA+H2O) and Continuous (C)(5d-PFM+OVA+PFM) treatment. At 7 and 15 days post-sensitization (dps) we analyzed: specific-IgE, specific-IgG and IL10 in serum. Non-specific-IgA, IL10 and IFNg in intestinal fluid (IF), and IL2+,IL4+ and IL10+ cells in the small intestine (SI). IgA+ cells in SI and bronchus and changes in the intestinal microbiota. At 7dps, specific-IgE decreased and IL10 increased in P and C groups compared with SC. IL10+ cells were increased for 7 and 15dps, however IL10 and IFNg release was not enhanced. The number of IL2+ cells was similar to the NC. IL4+ cells decreased for 15dps in treated groups but not for SC. For 7 and 15dps total s-IgA levels were higher than NC. IgA+ cells from SI and bronchus increased in C group. Changes in the intestinal microbiota were observed with diminution in enterobacteria, and increases of bifidobacteria populations in the treated groups. PFM was able to regulate IgE levels but not aOVA-IgG, by a decrease in the number of IL4+ cells in SI and increases of IL10. No variations in total s-IgA were found. The immune regulation observed for PFM in this allergy model could be mediated by the immune regulatory capacity exerted by bifidobacteria.