INVESTIGADORES
MALDONADO GALDEANO Maria Carolina
artículos
Título:
: ROLE OF VIABILITY OF PROBIOTIC STRAINS IN THEIR PERSISTENCE IN THE GUT AND IN MUCOSAL IMMUNE STIMULATION
Autor/es:
MARIA CAROLINA MALDONADO GALDEANO; GABRIELA PERDIGÓN
Revista:
Journal of Applied Microbiology
Editorial:
American Society for Microbiology
Referencias:
Año: 2004 vol. 97 p. 673 - 681
ISSN:
1364-5072
Resumen:
Probiotic bacteria viability is a requirement to be considered for the selection of probiotics at the intestinal level. Aims: To compare the interaction of viable and non viable probiotic bacteria with the intestine, and evaluate their behaviors in the intestinal mucosal immune system. Methods and results: Viable and non viable Lactobacillus strains were labeled with fluorescein isotiocyanate and administered to BALB/c mice. Samples from intestine were processed for fluorescence microscopy observation. L. casei was studied by transmission electronic microscopy (TEM) by using an antibody conjugated with colloidal gold. IgA+ and cytokine+ cells were determined in the intestine by immunofluorescence test. Antigens from viable L. casei and L. bulgaricus were observed in the Peyer’s patches and lamina propria (LP). Antigens from L. acidophilus were observed in nodule and LP of large intestine. Most fluorescent antigens from non viable bacteria were observed in the base of intestine. By TEM L. casei was observed adhered to the villi; it was found bacterial antigen in the cytoplasm of the enterocytes and in the intercellular spaces. Only viable bacteria stimulated the intestinal mucosal immune system. Conclusion: Bacteria viability was important to stimulate the mucosal immune response. Significance and Impact of the study: The short permanence time of the assayed LAB strain permit infer that continuous consumption of probiotics is safe besides important to guarantee an efficient immune response.