INVESTIGADORES
VIGNOLO Graciela Margarita
artículos
Título:
Combined effect of bacteriocins on the survival of various Listeria species en broth and meat system
Autor/es:
GM VIGNOLO,; J PALACIOS,; E FARÍAS, M; FERNANDO SESMA,; ULRICH SCHILLINGER,; WILHELM HOLZAPFEL,; G OLIVER,; GRACIELA MARGARITA VIGNOLO
Revista:
CURRENT MICROBIOLOGY
Editorial:
Springer
Referencias:
Lugar: New York; Año: 2000 vol. 41 p. 410 - 416
ISSN:
0343-8651
Resumen:
The antilisterial efficiency of three bacteriocins from lactic acid bacteria, lactocin 705
(produced by L. casei CRL705, 17000 AU/ml), enterocin CRL35 (produced by E. faecium CRL35,
17000 AU/ml), and nisin (2000 IU/ml), was tested in broth, individually and in combination againstL. casei CRL705, 17000 AU/ml), enterocin CRL35 (produced by E. faecium CRL35,
17000 AU/ml), and nisin (2000 IU/ml), was tested in broth, individually and in combination against
Listeria monocytogenes and Listeria innocua. Both Listeria species showed an initial decrease in
viable counts followed by the regrowth of the survivors after 1 h in the presence of each bacteriocin.
A greater antilisterial effect was observed when the bacteriocins were combined in pairs, maximal
inhibition being reached when nisin was involved. When a mix of the three bacteriocins was used,
no survivors were observed after 24 h of incubation. Similar results were obtained when the
bacteriocin combinations were tested in a meat system, indicating that the use of more than one LAB
bacteriocin in combination may be effective in preventing the spontaneous emergence of a bacteriocin-
resistant Listeria populationand Listeria innocua. Both Listeria species showed an initial decrease in
viable counts followed by the regrowth of the survivors after 1 h in the presence of each bacteriocin.
A greater antilisterial effect was observed when the bacteriocins were combined in pairs, maximal
inhibition being reached when nisin was involved. When a mix of the three bacteriocins was used,
no survivors were observed after 24 h of incubation. Similar results were obtained when the
bacteriocin combinations were tested in a meat system, indicating that the use of more than one LAB
bacteriocin in combination may be effective in preventing the spontaneous emergence of a bacteriocin-
resistant Listeria populationListeria population