INVESTIGADORES
VIGNOLO Graciela Margarita
artículos
Título:
Evaluation of anti-Listeria meat borne Lactobacillus for biofilm formation on selected abiotic surfaces
Autor/es:
PEREZ IBARRECHE, MARIANA; CASTELLANO, PATRICIA; VIGNOLO, GRACIELA
Revista:
MEAT SCIENCE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 96 p. 295 - 303
ISSN:
0309-1740
Resumen:
The ability ofmeat borne anti-Listeria Lactobacillus to form biofilms under different in vitro conditions and on abiotic
surfaces was investigated. Biofilm formation by the adhesion to polystyrene microtiter plates was determined,
this being higher for Lactobacillus curvatus CRL1532 and CRL705 and Lactobacillus sakei CRL1862. The
physicochemical properties of the cell surface were relatively hydrophilic and acidic in character; L. sakei
CRL1862 exhibiting the strongest autoaggregation. The adhesion of lactobacilli to stainless steel (SS) and
polytetrafluoroethylene (PTFE) supports at 10 °C was found to be maximal for L. sakei CRL1862 on SS after
6 days. When biofilm architecture was characterized by epifluorescence and SEM, L. sakei CRL1862 homogeneously
covered the SS surface while cell clusters were observed on PTFE; the extracellular polymeric substance
matrix adapted to the topography and hydrophilic/hydrophobic characteristics of eachmaterial. The feasibility of
L. sakei CRL1862 to formbiofilm onmaterials used inmeat processing highlights its potential as a control strategy
for Listeria monocytogenes biofilms.
