INVESTIGADORES
VIGNOLO Graciela Margarita
artículos
Título:
Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging application
Autor/es:
BLANCO MASSANI, MARIANA; MORANDO, PEDRO; VIGNOLO, GRACIELA; EISENBERG, PATRICIA
Revista:
LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013
ISSN:
0023-6438
Resumen:
Adsorption of bacteriocins produced by Lactobacillus curvatus CRL705, lactocin 705 (whose activity depends
upon complementation of two peptides, lac705a and lac705b) and bacteriocin/s with strong anti-Lactobacillus curvatus CRL705, lactocin 705 (whose activity depends
upon complementation of two peptides, lac705a and lac705b) and bacteriocin/s with strong anti-a and lac705b) and bacteriocin/s with strong anti-
Listeria activity, on a multilayer film was investigated. Lactocin 705 adsorption equilibrium at 30 C was
reached from 1 h of film contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass
adsorption maximum of 0.72 0.05 mg cm2 and a minimum inhibition concentration of 1 mg ml1. The
influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to
the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth
strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial
bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705b and lac705aactivity, on a multilayer film was investigated. Lactocin 705 adsorption equilibrium at 30 C was
reached from 1 h of film contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass
adsorption maximum of 0.72 0.05 mg cm2 and a minimum inhibition concentration of 1 mg ml1. The
influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to
the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth
strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial
bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705b and lac705afilm contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass
adsorption maximum of 0.72 0.05 mg cm2 and a minimum inhibition concentration of 1 mg ml1. The
influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to
the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth
strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial
bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705b and lac705a 0.05 mg cm2 and a minimum inhibition concentration of 1 mg ml1. The
influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to
the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth
strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial
bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705b and lac705afluence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to
the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth
strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial
bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705b and lac705afilm was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth
strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial
bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705b and lac705afluenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial
bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705b and lac705ab and lac705a
peptides complementation necessary for antimicrobial activity, while no interactions among impurities
and antilisterial bacteriocin/s during adsorption was suggested. Antilisterial bacteriocin/s activity on the
film was not influenced by lactocin 705 adsorption; conformational reorganization of adsorbed antilisterial
bacteriocin/s in the presence of lactocin 705 could allow the adsorption of both bacteriocins
while maintaining antilisterial antimicrobial activity. This study highlights the technological importance
of adsorption optimization to obtain effective antimicrobial food packaging systems.lm was not influenced by lactocin 705 adsorption; conformational reorganization of adsorbed antilisterial
bacteriocin/s in the presence of lactocin 705 could allow the adsorption of both bacteriocins
while maintaining antilisterial antimicrobial activity. This study highlights the technological importance
of adsorption optimization to obtain effective antimicrobial food packaging systems.