INVESTIGADORES
VIGNOLO Graciela Margarita
artículos
Título:
Molecular basis of the adaption of the anchovy isolate Lactobacillus sakei CRL1756 to salted environments through a proteomic approach.
Autor/es:
BELFIORE, CAROLINA; FADDA, SILVINA; RAYA, RAÚL; VIGNOLO, GRACIELA
Revista:
FOOD RESEARCH INTERNATIONAL
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013
ISSN:
0963-9969
Resumen:
Osmotic stress triggered by high salt concentrations results in the injury of starters during food processing.
The adaption of the anchovy isolate Lactobacillus sakei CRL1756 to salted environments in the presence of
osmoprotectants was analyzed by using a proteomic approach. Glycinebetaine (GB) pre-treated cells exposed
to 1.7 M NaCl+3 mM GB showed improved adaption achieving growth after a long lag phase while
non-proliferative cells in the salted medium without GB was observed. Comparative proteomic analyses of
the Lactobacillus cells grown in MRS+GB exposed or not to 10% salt revealed eighteen significantly regulated
proteins. Under hypertonic stress, decreased synthesis of four glycolytic enzymes (Fba, Pgk, Gpm5, Tpi) and
induction of other two (MleS, Pox2) related to an alternative energetic pathway, were registered. Proteins related
to general stress response and nucleotide metabolism were up-regulated. Noticeably, the induction of
DyP-type peroxidase, involved in iron transport, detoxification and oxidative stress, was observed for theLactobacillus sakei CRL1756 to salted environments in the presence of
osmoprotectants was analyzed by using a proteomic approach. Glycinebetaine (GB) pre-treated cells exposed
to 1.7 M NaCl+3 mM GB showed improved adaption achieving growth after a long lag phase while
non-proliferative cells in the salted medium without GB was observed. Comparative proteomic analyses of
the Lactobacillus cells grown in MRS+GB exposed or not to 10% salt revealed eighteen significantly regulated
proteins. Under hypertonic stress, decreased synthesis of four glycolytic enzymes (Fba, Pgk, Gpm5, Tpi) and
induction of other two (MleS, Pox2) related to an alternative energetic pathway, were registered. Proteins related
to general stress response and nucleotide metabolism were up-regulated. Noticeably, the induction of
DyP-type peroxidase, involved in iron transport, detoxification and oxidative stress, was observed for thebetaine (GB) pre-treated cells exposed
to 1.7 M NaCl+3 mM GB showed improved adaption achieving growth after a long lag phase while
non-proliferative cells in the salted medium without GB was observed. Comparative proteomic analyses of
the Lactobacillus cells grown in MRS+GB exposed or not to 10% salt revealed eighteen significantly regulated
proteins. Under hypertonic stress, decreased synthesis of four glycolytic enzymes (Fba, Pgk, Gpm5, Tpi) and
induction of other two (MleS, Pox2) related to an alternative energetic pathway, were registered. Proteins related
to general stress response and nucleotide metabolism were up-regulated. Noticeably, the induction of
DyP-type peroxidase, involved in iron transport, detoxification and oxidative stress, was observed for theLactobacillus cells grown in MRS+GB exposed or not to 10% salt revealed eighteen significantly regulated
proteins. Under hypertonic stress, decreased synthesis of four glycolytic enzymes (Fba, Pgk, Gpm5, Tpi) and
induction of other two (MleS, Pox2) related to an alternative energetic pathway, were registered. Proteins related
to general stress response and nucleotide metabolism were up-regulated. Noticeably, the induction of
DyP-type peroxidase, involved in iron transport, detoxification and oxidative stress, was observed for thefication and oxidative stress, was observed for the
first time in lactic acid bacteria under osmotic constraint. Our results demonstrate that GB played a significant
role in protecting L. sakei CRL1756 against salt stress. Indeed, GB commonly found in marine fish may be used
by the starter culture cells ensuring their robustness in salted-anchovy based products.rst time in lactic acid bacteria under osmotic constraint. Our results demonstrate that GB played a significant
role in protecting L. sakei CRL1756 against salt stress. Indeed, GB commonly found in marine fish may be used
by the starter culture cells ensuring their robustness in salted-anchovy based products.L. sakei CRL1756 against salt stress. Indeed, GB commonly found in marine fish may be used
by the starter culture cells ensuring their robustness in salted-anchovy based products.
