Nasal priming with peptydoglican of probiotic strains can improve the adaptive immune response in malnourished mice

KOLLING YANINA; SALVA SUSANA; VILLENA JULIO; ALVAREZ SUSANA

Mar del Plata

Congreso; Reunión Conjunta Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

Reunión Conjunta Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE)

Lactobacillus rhamnosus CRL1505 peptidoglycan (Pg05) increases the infection resistance against Streptococcus pneumoniae (Sp) and improves innate immune response in immunocompromised-malnourished mice. The aim of this work was the comparative study of the effect of three peptidoglycans on the behavior of cellular and humoral components of adaptive immune response against Sp. In addition to Pg05, peptidoglycans from probiotic L. plantarum CRL1506 (Pg06) and the non-immunomodulatory control L. rhamnosus CRL534 (Pg534) were evaluated.Weaned mice were malnourished with a protein-free diet for 21d and then received a balanced diet during 7d (BD group) or BD with nasal supplementation of Pg05, Pg06 or Pg534 during the last 2d. On d8, all groups were infected with Sp. The T and B cell populations and the production of Th1/Th2 cytokines and specific antibodies were studied. After infection, the number of thymus T cells (CD4-CD8-, CD4-CD25+, CD4+CD8+) increased in the BD group, but the values of the Pg05 group were higher than those of BD group. In the spleens and the lungs, CD3+CD4+ and CD3+CD8+ cells augmented, but only Pg05 induced a bigger increase than the BD groups. The Pg05 showed a rise in the values of Th1/Th2 cytokines in broncho-alveolar lavages (BAL) and serum at 10dpi compared with the BD group. The Pg05 group showed an increase of pro-pre B and immature B cells in bone marrow as well as mature B cells in spleen and lung. Moreover, the Pg05 mice had higher levels of BAL and serum anti-pneumococcal antibodies and improved opsonophagocytotic activity than the others groups. These particular findings demonstrate that immunomodulatory effect of the Pg05 is a strain specific property and opens interesting questions regarding the molecular differences between peptidoglycans. Cellular fractions could be an interesting alternative as mucosal adjuvants, especially in immunocompromised hosts in which the use of live bacteria might be unsafe.