INVESTIGADORES
ALVAREZ Gladis Susana
congresos y reuniones científicas
Título:
Promotion of myelopoietic cells expansion and differentiation induced by Lactobacillus rhamnosus CRL1505 in emergency myelopoiesis
Autor/es:
GRAMAJO LÓPEZ ANDRÉS; KOLLING YANINA; VILLENA JULIO; ALVAREZ SUSANA; SALVA SUSANA
Lugar:
Tucumán
Reunión:
Simposio; V International Symposium on Lactic Acid Bacteria: Benefitting from Lactic Acid Bacteria - Progress in Health and Food; 2016
Institución organizadora:
Centro de Referencia para Lactobacilos
Resumen:
Emergency myelopoiesis is the inflammation-induced hematopoiesis to replenish myeloid cells in the periphery, which is critical to control the infection with pathogens. Dietary supplementation with probiotic Lactobacillus rhamnosus CRL1505 was demonstrated to be an interesting alternative to improve steady-state and emergency myelopoiesis. This, in turn, led to resistance against pathogens in immunosuppression states caused by chemotherapy. While the viability of the lactic acid bacteria (LAB) is an important factor to achieve optimal protective effects, it is possible to stimulate immunity in immunosuppressed hosts using non-viable LAB. The aim of this work was to evaluate whether L. rhamnosus CRL1505 was able to improve emergency myelopoiesis induced by Streptococcus pneumoniae (Sp) infection. Adult Swiss-mice were orally treated with viable or non-viable L. rhamnosus CRL1505 (Lr05V or Lr05NV, respectively) during five consecutive days. On day 6, lactobacilli-treated and untreated control mice received one dose intraperitoneally of cyclophosphamide (Cy 150 mg/kg). On day 3 post-Cy injection, mice were nasally challenged with Sp (107 UFC/mice). Innate and myelopoietic responses were evaluated after the pneumococcal challenge. Before infection, Cy impaired myelopoiesis, but viable or non-viable LAB were able to protect the mice against damage induced by chemotherapy on hematopoiesis. After infection, the Cy group showed a high susceptibility to pneumococcal infection; however, lactobacilli treatments were able to significantly reverse those effects (p