INVESTIGADORES
ALVAREZ Gladis Susana
congresos y reuniones científicas
Título:
The toll-like receptor family protein RP105/MD-1 complex is involved in the immunoregulatory effect of Lactobacillus plantarum N14 exopolysaccharides
Autor/es:
MUROFUSHI Y; VILLENA JULIO; MORIE K; HASHIGUCHI K; YOSHIDA M; ALVAREZ SUSANA; SAITO TADAO; KITAZAWA HARUKI
Lugar:
Tucumán
Reunión:
Simposio; IV International Symposium on Lactic Acid Bacteria - Food, Health and Applications; 2013
Institución organizadora:
Centro de Referencia para Lactobacilos. CERELA-CONICET
Resumen:
THE TOLL-LIKE RECEPTOR FAMILY PROTEIN RP105/MD-1 COMPLEX IS INVOLVED IN THE IMMUNOREGULATORY EFFECT OF Lactobacillus plantarum N14 EXOPOLYSACCHARIDES The radioprotective 105 (RP105)/MD-1 complex is a member of the Toll-like receptor (TLR) family of proteins. It was reported that this complex cooperates with the essential lipopolysaccharide (LPS) receptor TLR4/MD-2 complex and plays a crucial role in LPS responses by immune cells. Previously, we cloned the cDNAs encoding porcine RP105 and porcine MD-1 from Peyer´s patches (PP) of adult swine and demonstrated in experiments with cells cotransfected with RP105 and MD-1 (HEKRP105/MD-1 cells) that Lactobacillus plantarum N14 (LP14) strongly activate NF-kB and induce the expression of various cytokines via RP105. In this work, we evaluated the immunoregulatory capacities of extracellular phosphopolysaccharides (EPS) from LP14 in intestinal epithelial and immune cells from pigs. In addition, we aimed to gain insight in the knowledge of the mechanisms involved in the immunoregulatory effect of EPS by studying their capacity to stimulate the RP105/MD-1 complex, TLR4 and TLR2. EPS from LP14 were fractionated into neutral (NPS) and acidic (APS) EPS by anion exchange chromatography. Our results showed that APS from LP14 is able to increase the mitogenic activities of PP and mesenteric lymphoid node (MLN) immune cells in a dose dependent manner while NPS strongly stimulated mitogenic activity of MLN in low concentrations. In addition, APS upregulated the expression of IL-6 and IL-8 in immune cells from MLN and PP and in porcine intestinal epithelial (PIE) cells while NPS significantly increased TGF-β levels in both PP immune cells and PIE cells (p