Effect of different routes of administration of Lactobacillus rhamnosus CRL1505 on emergency granulopoiesis and respiratory innate immune response in protein-malnourished mice

SUSANA SALVA; HERRERA HÉCTOR MATÍAS; JULIO VILLENA; NATALIA BARBIERI; GABRIELA MARRANZINO; GRACIELA AGÜERO; SUSANA ALVAREZ

Los Cocos, Córdoba

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Inmunología (SAI); 2013

Sociedad Argentina de Inmunología

This work studied the effect of protein malnutrition on the hemato-immune response to the challenge with Streptococcus pneumoniae (Sp) and evaluated whether the dietary recovery with supplemental probiotics has a beneficial effect in that response. Malnourished mice received balanced conventional diet (BD) during 7d (B group) or BD with oral Lactobacillus rhamnosus CRL1505 supplementation (108 cells/mouse/d) during last 5d of the treatment (LO group); or nasal supplementation during last 2d of the treatment (LN group). On d8, these groups, well-nourished (WC) and malnourished (MC) controls, were infected nasally with Sp (105 cells/mouse). Leucocytes numbers and phagocytes activity, Gr1 and CD34 expression and the levels of IL-6, TNF-α, IL-1β, GM-CSF, IL-10 and IFN-γ were studied in broncho-alveolar lavages and blood. In addition, leucocytes numbers and expression of Gr1, CD34, CXCL12, GM-CSF, IL-1β and SCF were analyzed in bone marrow (BM). Protein-malnutrition significantly impaired innate immunity and emergency myelopoiesis in response to pneumococcal infection, since reduced recruitment and activity of neutrophils and reduced levels of proinflammatory cytokines were detected in lungs of MC mice (p