Lactobacillus rhamnosus CRL1505 modulate the inflammation-coagulation response triggered by nasally administered poly(I:C)

HORTENSIA ZELAYA; ERIKO CHIBA; GABRIELA MARRANZINO; SUSANA ALVAREZ; HARUKI KITAZAWA; GRACIELA AGÜERO; JULIO VILLENA

Los Cocos, Córdoba

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Inmunología (SAI); 2013

Sociedad Argentina de Inmunología

During infectious process, there is extensive cross-talk between the innate immune and hemostatic systems, as both are involved in host?s defense. The aim of this study was to evaluate the effect of orally administered Lactobacillus rhamnosus CRL1505 (Lr1505) on the immuno-coagulative response after the intranasal challenge with the viral pathogen associated molecular pattern poly(I:C). Six-week old BALB/c mice were orally treated with Lr1505 (108 cells/mouse/day) during 5 consecutive days. Lr1505-treated mice and untreated control were nasally challenged with 250 μg of poly(I:C) (equivalent to 10 mg/kg body weight). Mice received three doses of poly(I:C) with 24 hours rest period between each administration. Respiratory and systemic immune-coagulative response were studied 12 hours after the last administration of poly(I:C). Nasal challenge with poly(I:C) increased the levels of blood and respiratory proinflammatory mediators and induced the recruitment of inflammatory cell into the airways. In addition, poly(I:C) challenge increased prothrombin activity (PT) in plasma and thrombin/antithrombin complexes (TATc) and von Willebrand Factor (vWF) levels in plasma and lungs, indicating an activation of coagulation. These changes induced a significant damage in lung tissue. Lr1505-treated mice showed significantly reduced TNF-α, IL-8 and MIP-1α and increased IL-10 levels in lung and serum when compared to controls (p