INVESTIGADORES
ALVAREZ Gladis Susana
congresos y reuniones científicas
Título:
Lactobacillus casei ADDITION TO A REPLETION DIET INDUCE MODIFICATIONS ON SPLEEN AND BONE MARROW CELL POBLATIONS
Autor/es:
SALVA SUSANA; ALVAREZ SUSANA; AGÜERO GRACIELA
Lugar:
Tafí del Valle. Tucumán
Reunión:
Jornada; XXII Jornadas Científicas de la Asociación de Biología de Tucumán; 2005
Institución organizadora:
Asociación de Biología de Tucumán
Resumen:
The addition of Lactobacillus casei (Lc) to a repletion diet, induce proliferation and differentiation of bone marrow (BM) myeloid and lymphoid series, in malnourished mice (UM). Lc administration accelerates the clearance of pneumococci during a respiratory infection in UM. Aim: To study comparatively cell populations of spleen and BM, during the repletion with or without Lc; and to analyze cellular mobilization in order to explain the possible mechanisms involucrate in the pathogen clearence. UM were feed 7, 14 or 21 days with: balanced diet (BD) or BD with supplemental Lc on the last 2 days of each repletion period (BD+Lc). After treatments, we determined: body weight (bw) increase, spleen and thymus weight, hemograma, % of reticulocytes, % of peroxidase positive cells (P+) in peripheral blood (PB) and BM, CD4+, CD8+ and CD19+ cells in spleen and BM. Results: All diet increased the bw, spleen and thymus weight in UM. Leukocyte counts were normalized with 21d of both diets.  Malnutrition induced an important decrease in the % of reticulocytes in PB. At 7d, both diets increase this parameter which was higher with BD+Lc. The % CD19+ cells in spleen and BM were normalized with 7d BD+Lc and 14d BD. CD8+ cells in spleen and BM reached the normal values with 14d BD and 14d BD+Lc. BM CD4+ cells were normalized at 7d with both diets, but in spleen the normalization happen at 7d just with BD+Lc, while with BD was normalized at 14d. Conclusion: The addition of L. casei to the repletion diet, accelerates the normalization of cellular populations in the spleen and BM. The increase of peroxidase activity and the number of lymphoid cells in the spleen, would explain the more efficient cellular mobilization in the presence of the infection.