INVESTIGADORES
ALVAREZ Gladis Susana
congresos y reuniones científicas
Título:
Effect of L. lactis intranasally or orally administered in pneumococcal respiratory infection. Studies on systemic and local immune cells
Autor/es:
MEDINA MARCELA; SALVA SUSANA; VINTIÑI ELISA; ALVAREZ SUSANA
Lugar:
Córdoba. Argentina
Reunión:
Congreso; VII Cong. Latinoamericano de Inmunología. ALAI; 2005
Institución organizadora:
Sociedad Latinoamericana de Inmunología
Resumen:
Administration of Lactococcus lactis NZ 9000 during 5 days (d) by intranasal (LLIN) or oral (LLO) routes reduces Streptococcus pneumoniae (Spn) cells count in lung of infected mice; only LLIN treatment prevented its dissemination. Objective:to evaluate the effect of LLIN and LLO administration on immune cells of blood (B), bone marrow (BM) and bronchoalveolar (BAL) and peritoneal (PL) lavages. Mice received LLIN or LLO, at the end of treatments these groups and control mice(C) were intranasally challenged with 106CFU Spn/mouse.Assays were performed before(0d) and after challenge (during 15d post-infection(dpi)):a)Total and differential leukocytes(L) counts in B and BAL b)BM cells % differential count c)Number of BM CD4+,CD8+ and CD19+cells by IF d)NBT test for phagocytes activity in BAL and PL.After infection, neutrophils number (cells.105/ml) in BAL were increased for LLIN (10dpi:C=1,3±0,7 LLIN=2±0,4) and LLO (10dpi:C=1,3±0,7 LLO=1,8±0,4).LLIN increased %NBT+cells in BAL(0d:C=25,8±0,8 LLIN=43,5±1,2 1dpi:C=63,8±1,1 LLIN:79,3±0,9) and LP (0d:C=23,7±1,0 LLIN=37,9±0,6 1dpi:C=38,9±1,3 LLIN=54,9±1,1).After challenge, %BM cells in the non-mitotic pool were increased in LLIN and LLO compared with C (15dpi:C=53±2 LLIN=75±1 LLO=73±2).LLIN and LLO increased CD4+cells (cells/10 fields)(10dpi:C=2,1±0,3 LLIN=5,2±0,2 LLO=3,4±0,4) and CD8+cells (10dpi:C=1,3±0,3 LLIN=3,4±0,6 LLO=3,1±0,4) in BM. Only LLIN increased CD19+cells (10dpi:C=4,1±0,5 LLIN=5,9±0,6).LLIN was more effective than LLO to induce myeloid differentiation and myeloid-lymphoid proliferation in BM and mobilization to other compartments during pneumococcal infection