INVESTIGADORES
ALVAREZ Gladis Susana
congresos y reuniones científicas
Título:
Lactobacillus casei reduce las alteraciones histológicas de médula ósea inducidas por ciclofosfamida
Autor/es:
HERRERA MATÍAS; VILLENA JULIO; SALVA SUSANA; BARBIERI NATALIA; ALVAREZ SUSANA
Lugar:
Tafí del Valle. Tucumán. Argentina
Reunión:
Jornada; XXVI Jornadas Científicas de la Asociación de Biología de Tucumán; 2009
Institución organizadora:
Asociación de Biología de Tucumán
Resumen:
We evaluated the effect of L. casei CRL431 (Lc) administration on bone marrow (BM) histological and cytomorphological changes induced by cyclophosphamide (CF) in mice. A group of mice was fed Lc for 2 d; this group and the untreated control mice (C) received an intraperitoneal injection of CF (150 mg/ kg). Before and after CF injection we carried out total and differential cell counts in BM and histological studies. CF administration significantly decreased BM cell numbers in both groups, affecting mainly non-mitotic pool cells. Lc mice showed a lower decrease in BM leukocytes and higher levels of mitotic pool cells when compared to the C group. Histological studies showed that CF decreased BM myeloid and lymphoid series (1-3d post-CF). In the C group, eritroblasts, adipocytes and megakaryocytes were the predominat cells. In addition, accumulation of acidophilic material and sinusoids alteration were observed (3-4 d post-CF). The BM architecture was recovered on d 10. In mice treated with Lc the myelo/erythroid ratio was higher than in the C group and myeloid, lymphoid and mature red cells were less affected than in the C group (1-3 d post-CF). In the Lc treated mice, the BM architecture was normalized on d 7. According to these results, the preventive administration of probiotic lactic acid bacteria could be able to reduce BM histological alterations associated with chemotherapy treatments.