INVESTIGADORES
ALVAREZ Gladis Susana
congresos y reuniones científicas
Título:
Effects of exogenous sexual hormones on the vaginal microbiota and immune cells in mice
Autor/es:
DE GREGORIO PRISCILA; JUÁREZ TOMÁS MARÍA SILVINA; SALVA SUSANA; SANTOS VIVIANA; ALVAREZ SUSANA; NADER-MACÍAS MARÍA ELENA FÁTIMA
Lugar:
Puerto Madryn. Chubut
Reunión:
Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. SAIB; 2010
Institución organizadora:
SAIB
Resumen:
The effect of progesterone (P) and estradiol (E) on several organs of
an experimental animal model was evaluated. 2-months-old female
BALB/c mice were inoculated with (P) (day -5) or E (day -2) to
induce and maintain the different stages of the estrous cycle. The
samples taken on days 0, 2, 6 and 8 post-injection of hormones
were: blood, vaginal fluid (VF), vagina, spleen and bone marrow
(BM). Most of the animals remained in diestrous-metaestrous
stages until day 6 post-P-injection or in proestrous-estrous stages
until day 8 post-E-injection. The numbers of enterobacteria,
staphylococci and lactic acid bacteria in VF were higher in
estrogenized animals. In VF, myeloid population (Gr-1+) was
dominant compared to T (CD3+) and B (B220+) lymphoid
populations, determined by flow cytometry. On most of days, the
Gr-1+ cells of VF were higher in estrogenized mice. The hormonal
treatments did not produce significant differences in the myeloid
and T cell populations of BM or spleen. Only in spleen, a significant
increase of mature B cells (B220HighCD24Low) and a decrease of
immature B cells (B220LowCD24High) was observed in P-inoculated
mice compared with those E-inoculated. These results allow the
selection of the most suitable experimental conditions to further
evaluate the mechanisms of interaction between beneficial vaginal
lactobacilli and the host in a murine experimental model.