Naringenin interferes with the dispersal of Staphylococcus aureus biofilms.

LOMBARTE SERRAT A; DOTTO C; SULIGOY M; CERVINI BOHM G; SAENZ D; SORDELLI D; GIACOMODONATO M; BUZZOLA F

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias. LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica SAIC 2017; 2017

SAIC

Staphylococcus aureus biofilms are recalcitrant to antibiotic treatment leading to chronic infections. The final benefit to the biofilm development is the potential for seeding dispersal or cellular detachment. The dispersal mechanism requires an active agr quorum sensing system and occurs in a protease-dependent manner. Naringenin (N) is a natural flavanone with antioxidant and antimicrobial properties. The aim of this study was to investigate the effect of N on S. aureus biofilm. S. aureus AR411 (osteomyelitis isolate related to USA300 clone) and SH1000 laboratory strain were used. Citotoxicity using LM2 cell line was determined by MTT assay. Bacteria were treated with N before biofilm formation takes place (prior-to-exposure) and 24 h after biofilms were formed (post-exposure). Biofilms were spectrophotometrically quantified. Inhibition of agr system was monitored fluorometrically. Proteolytic activity was assessed using milk agar plates. N (50 μg/ml) did not affect the epithelial cells viability. The ability to form biofilm by AR411 strain (prior-to exposure) significantly diminished in presence of N (AR411: 3.270.11 vs AR411+N: 1.640.08, p