INVESTIGADORES
GIACOMODONATO Monica Nancy
congresos y reuniones científicas
Título:
Dam enzyme participates in the regulation of biofilm production in Salmonella enterica serovar Typhimurium.
Autor/es:
MARÍA DEL ROSARIO AYA CASTAÑEDA, SEBASTIÁN HERNÁN SARNACKI, MARIÁNGELES NOTO LLANA, MÓNICA NANCY GIACOMODONATO AND MARÍA CRISTINA CERQUETTI.
Reunión:
Conferencia; 5th International Student Conference on Microbial Communication; 2015
Resumen:
On the bacterial world, existence within surface-associated structured multicellular communities may be the rule, rather than the exception [1]. It has been demonstrated that several serovars of S. enterica, including Typhimurium, are able to attach to and form biofilms on a variety of surfaces, and this ability has been implicated in the persistence of non-typhoidal Salmonella in the environment and in industrial, veterinary and medical settings [2]. Non-typhoidal salmonellosis is an important foodborne disease of worldwide economic significance. Salmonella Typhimurium (ST) can be found in some laying flocks in the European Union (EU) and in earlier years, has been associated with outbreaks involving contamination of egg contents [3]. Here, we studied the involvement of Dam methylations in biofilm production in S.T strains. The ability to generate biofilms and the expression of curli and cellulose were analyzed in wild type and mutant strains. Also, the expression of five main genes that are involved on biofilm production -csgD, csgA, bcsA, adrA and bapA- was determined by qPCR, in different culture conditions. We observed that dam-defective strains of S.T are impaired in their capacity to develop biofilms and that the production of cellulose and curli are affected, mainly curli production.  Our results suggest that changes in gene expression required for biofilm production are finely regulated by Dam methylation. Thus, methylations could modulate csgD expression and up regulate the expression of factors related with biofilm production, including biofilm-associated proteins, curli and cellulose. Biofilm production plays an important role in bacterial infectious process and this study contributes to the understanding of biofilm regulation in Salmonella.