Effect of TGF-ß1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells

RODRIGUEZ, TM; SALDIAS, A; IRIGO, M; VELASCO ZAMORA, J; PERONE, MJ; DEWEY, RA

Stem Cells Translational Medicine

AlphaMed Press

Año: 2015

2157-6564

Adipose tissue is an attractive source of mesenchymal stromal cells (MSCs) owing to the relative easeof obtaining large volumes with more MSC abundance compared with other sources. Increasing evidencesupports the fact that trophic factors secreted by MSCs play a pivotal therapeutic role. Severalstrategies in regenerative medicine use MSCs, mainly exploiting their immunosuppressive effect andhoming capacity to sites of damage. Transforming growth factor-b1 (TGF-b1) is a pleiotropic cytokinethat, depending on the cell niche, can display either anti-inflammatory or proinflammatory effects.TGF-b1 expression increases in various tissues with damage, especially when accompanied by inflammation.Thus, we analyzed the effect of TGF-b1 on the secretion by adipose-derived mesenchymalstromal cells (ASCs) of a panel of 80 cytokines/chemokines using an antibody array. To avoid a possibleeffect of fetal bovine serum (FBS) on ASCs secretion, we performed our analysis by culturing cells inFBS-free conditions, only supplemented with 0.1% of bovine serum albumin. We report the cytokineprofile secreted by ASCs. We also found that TGF-b1 exposure modulates 8 chemokines and 18 cytokines,including TGF-b1 and -b2, and other important cytokines involved in immunosuppression, allergicresponses, and bone resorption.