Development of carbohydrate functionalized magnetic nanoparticles for aminoglycosides magnetic solid phase extraction

CONTIN, MARIO DANIEL; J. E. QUINSAAT; NEGRI, R. MARTÍN; TRIPODI, V.D.; OPRIS, DORINA; D´ACCORSO, N.

ANALYTICA CHIMICA ACTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2019

0003-2670

Magnetic nanoparticles decorated with galactose and galactitol (Fe3O4@SiN-galactose and Fe3O4@SiN-galactitol) were synthesized and employed as sorbent in a magnetic solid phase extraction (MSPE) procedure prior the analysis of aminoglycosides (AGs) in honey samples by LC-MS/MS. AGs are broad spectrum antibiotics, characterized by aminosugars, widespread used in therapeutic and veterinary applications. AGs can be found in the environment and food of animal origin. Fe3O4@SiN-galactose and Fe3O4@SiN-galactitol were synthesized via cooper catalyzed alkine azide cycloaddition and characterized by electronic microscopy, thermo gravimetric analysis, infrared spectroscopy and performing magnetization curves. The nature of the loading (acetonitrile:water, 50:50 v/v) and elution solution (formic acid 190 mM) were studied in order to optimize the MSPE. Quantitative difference between MSPE with Fe3O4@SiN-galactose and MSPE with Fe3O4@SiN-galactitol in terms of recovery were found. The final optimized method using Fe3O4@SiN-galactose and Fe3O4@SiN-galactitol was applied in the determination of AGs in honey being MSPE performance with Fe3O4@SiN-galactitol superior than MSPE with Fe3O4@SiN-galactose. The limits of quantification were between 2 to 19 µg Kg-1 for amikacin, dihydrostreptomycin, tobramicyn and gentamycin. A good correlation between predicted and nominal values of AGs in honey was found (trueness from 84 % to 109%) except for dihydrostreptomycin at low level. This MSPE procedure requires a minimum amount of sorbent (1 mg) , sample (0.2 g) and can be accomplish in a short time.