DIFFERENT KINDS OF POLYMERIC NANOPARTICLES WITH APPLICATION IN ANTIBACTERIAL PHOTOTHERMAL THERAPY

S. BONGIOVANNI ABEL; M.S. DARDANELLI; C.A. BARBERO; C.R. RIVAROLA; E. YSLAS

Mar del Plata

Otro; SAIC-SAI-SAFIS 2018; 2018

SAIC

Due to the high resistance to antibiotics developed by different bacteria, new methods are currently required for the treatment of infections generated by these pathogens. Among the alternative therapeutic modalities to the conventional ones, photothermal therapy (PTT) stands out. It is based on the absorption of radiation (e.g. NIR light) by molecules or materials and the subsequent generation of heat in the surrounding medium when transforming the incident energy. This phenomenon can be exploited to cause the death of the pathogen. (1) In this sense, with the rise of nanotechnology, different materials have been synthesized and characterized to be applied in PTT. In this work, we demonstrate how is possible to use nanoparticles based on conducting polymers (NP-PANI) generated by different methods (oxidative polymerization from monomer (2) and solvent displacement -nanoprecipitation ?(3)) to decrease via PTT the viability of pathogenic bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus, both responsible for a large number of nosocomial infections. The results show that in both cases more than 80% of the viability is reduced, regardless of the kind of nanoparticle, there being an antibacterial synergistic effect given by the contribution of the nanoparticulate conducting polymer and the action of the NIR light. The PTT phenomena was corroborated by techniques such as fluorescence microscopy and DNA fragmentation. (1)Referencias:1. S. Bongiovanni Abel et al. Biomed. Phys. Eng. Express 4, 2018, 045037.2. S. Bongiovanni Abel et al. Nanotechnology 25 (49), 2014, 495602.3. S. Bongiovanni Abel et al. Nanotechnology 29, 2018, 125604.