Photothermal therapy of the pathogenic bacteria with polyaniline nanoparticles

L.A. GALLARATO; S. BONGIOVANNI ABEL; M.S. DARDANELLI; C. A. BARBERO; E.I. YSLAS

Mar del Plata

Congreso; - SAIB - 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2015

Increases in the prevalence of antibiotic resistant bacteria require new approaches for the treatment of infectiousbacterial pathogens. It is now clear that nanotechnology has driven a new approach in the using nanoparticles todestroy pathogenic bacteria. A new therapeutic modality is called photothermal therapy. This therapy is based in theuse of conductive nanoparticle that absorbed in the range of near-infrared (NIR) radiation and can transfer this energyinto the surrounding environment as heat that could cause irreparable cellular damage. In this work were performedexperiments to determine if these polyaniline nanoparticle (PANI-Np) dispersed in polivinylpirrolidone (PVP) incombination with NIR irradiation could be used to destroy Pseudomonas aeruginosa (P. aeruginosa) andStaphylococcus aureus (S. aureus) cells. The bacteria were incubated with PANI-Np and irradiated during 15 minwith focused laser (785nm, 500mW) and post-treatment was observed bacterial damage and was verified by viabilitytesting. In both microorganisms the viability reduction was about 10%, compared to control conditions. Thismechanism of cell death was observed by DNA fragmentation. These results reveal that the PANI-Np, following NIRexposure can be used for killing bacteria because was observed a significant decrease in cell viability triggering celldeath.