Synthesis of Polyaniline (PANI) and Functionalized Polyaniline (F-PANI) Nanoparticles with Controlled Size by Solvent Displacement Method. Application in Fluorescence Detection and Bacteria Killing by Photothermal Effect.

S. BONGIOVANNI ABEL; E.I. YSLAS; C. R. RIVAROLA; C. A. BARBERO

NANOTECHNOLOGY

IOP PUBLISHING LTD

Año: 2018 vol. 29 p. 125604 - 125615

0957-4484

Polyaniline nanoparticles (PANI-NPs) were easily obtained applying the solvent displacement method by using Nmethylpyrrolidone(NMP) as good solvent and water as poor solvent. Different polymers such as polyvinylpyrrolidone (PVP),chondroitin sulfate (ChS), polyvinyl alcohol (PVA), and polyacrylic acid (PAA) were used as stabilizers. Dynamic Light Scattering andScanning Electron Microscopy corroborated the size and morphology of the formed NPs. It was demonstrated that the size ofnanoparticles could controlled by setting the concentration of PANI in NMP, the NMP to water ratio, and the stabilizer´s nature. Thefunctionalization and fluorescence of NPs were checked by spectroscopic techniques. Since polyaniline show only weak intrinsicluminescence, fluorescent groups were linked to the polyaniline chains prior to the nanoparticle formation using a linker. Polyanilinechains were functionalized by nucleophilic addition of cysteamine trough the thiol group thereby incorporating pendant primary aliphaticamine groups to the polyaniline backbone. Then, dansyl chloride (DNS-Cl), which could act as an extrinsic chromophore, was conjugatedto the amine pendant groups. Later, the functionalized polyaniline was used to produce nanoparticles by solvent displacement. Theoptical and functional properties of fluorescent nanoparticles (F-PANI-NPs) were determined. F-PANI-NPs in the conductive state (pH