EVALUATION OF BIOENGINEERED ACELLULAR BONE SUBSTITUTES IN THE HEALING OF CRITICAL INJURIES

KOKUBU, SABRINA; KOKUBU, GABRIEL ; HAGELIN, KARIN; MONTANER, ALEJANDRO; BALAÑA, MARIA EUGENIA; LEIRÓS, GUSTAVO JOSÉ

Buenos Aires

Congreso; Reunion Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de investigacion Clinica

Bioengineering strategies will allow the development of newapproaches for the treatment of bone loss. Biocompatible matricescontaining bio-modulating molecules, involved in cell signaling andin cell-extracellular matrix interaction, are a promising alternative totreat critical bone injuries.The aim of this study was to evaluate a bone substitute, constitutedby a collagen matrix embedded with BMP-2 or oligonucleotide(ODN) 504, in the healing of critical bone lesions in a rat calvariamodel.Bone substitutes were generated using Hellitape (collagen type Imembrane) containing BMP-2 500ng/ml or Biopad (collagen typeI sponge) containing oligonucleotide (ODN) 504 1mg/ml. Theseconstructs and their specific controls (matrices) were applied tosurgery-generated critical calvaria lesions (10 male Wistar rats pergroup). An absolute control group (untreated lesion) was also performed.Half of the animals in each group were sacrificed after 10days and another half after 40 days post-surgery. The analysis of theremaining bone lesion area was performed using Image-J softwarefrom X-rays images.Ten days post-surgery, radiographic studies did not show significantdifferences in wound closure areas between groups. Even if40 days post-surgery, all groups showed a significant decrease inthe lesion area compared to 10 days post-surgery, only the Hellitape-BMP2 group and its control showed a significantly improved closureof the lesion. In particular, the Hellitape-BMP2 group showedthe greatest closure when compared to its control. Our results supportthe Hellitape-BMP2 construct as a promising bone substituteto treat critical bone lesions. However, a greater number of animalsshould be analyzed.