INVESTIGADORES
BALAÑA maria eugenia
congresos y reuniones científicas
Título:
Hair follicle neogenesis in composite skin substitutes using human adult cells
Autor/es:
STELLA IY; ANA GABRIELA KUSINSKY; MARIA LIA CASTELLANOS; GUSTAVO JOSÉ LEIRÓS; BALAÑA MARÍA EUGENIA
Lugar:
Copenhagen
Reunión:
Congreso; 44th Annual Meeting of the European Society for Dermatological Research (ESDR).; 2014
Institución organizadora:
European Society for Dermatological Research (ESDR).
Resumen:
Tissue-engineered skin represents a useful strategy for the treatment of deep skin injuries. The growth of hair follicles in vitro or after grafting remains a major challenge. The dermal-epidermal composites are skin substitutes comprised of dermal fibroblasts (DF) embedded in a matrix overlaid with keratinocytes. Hair Follicle Stem Cells (HFSC) contributes to hair follicle regeneration and wound repair. Dermal papilla cells (DPC) signaling orchestrate hair follicle morphogenesis and regeneration. The purpose of the work was to compare DPC and DF as dermal component in a composite skin with human HFSC using a porcine acellular dermal matrix (ADM) as scaffold. The HFSC-DF and HFSC-DPC constructs as well as the ADM alone were grafted in nude mice. In this work, we demonstrated that graft-take and tissue remodeling in nude mice were favored in DPC-containing composite skin. At day 14 after grafting, HFSC-DPC constructs showed the highest number of neovessels mainly located immediately bellow of epidermis probably supporting its survival. Concordantly, HFSC-DPC showed an earlier beginning of matrix remodeling process whereas ADM alone or HFSC-DF showed a more intense inflammatory response. A stable population of p63-positive cells was maintained in HFSC-DPC in vivo, while in HFSC-DF ones a significant reduction of these cells was observed. Furthermore, only in grafted HFSC-DPC constructs structures reminiscent of embryonic development of the hair follicle were observed from 14 days after grafting. These structures revealed, by immunohistochemical analyses, cells of human origin (HLA I), presence of precursor epithelial cells (p63) and expression of a hair differentiation marker (K6hf). These observations suggest an incipient hair follicle neogenesis inside the remodeling ADM. Taken together our results show DPC and HFSC as promising cellular components for a permanent skin substitute.