Evaluation of bio-engineered composite skin substitute using Human Dermal Papilla Cells and

GUSTAVO JOSÉ LEIRÓS; HUGO DRAGO; SILVIA BOSSI; FLAVIO STURLA; ANA GABRIELA KUSINSKY; MARIA LIA CASTELLANOS; INES STELLA; MARIA EUGENIA BALAÑA

Congreso; 42nd Annual European Society for Dermatological Research Meeting; 2012

European Society for Dermatological Research

  Evaluation of bio-engineered composite skin substitute using Human Dermal Papilla Cells and Hair Follicle Stem Cells in immunocompetent mice GJ Leirós,1 H Drago,2 S Bossi,2 S Flavio,2 AG Kusinsky,1 L Castellanos,1 I Stella3 and ME Balañá11 H Drago,2 S Bossi,2 S Flavio,2 AG Kusinsky,1 L Castellanos,1 I Stella3 and ME Balañá1 1 Fundacion Pablo Cassará, Instituto de Ciencia y Tecnología César Milstein (CONICET), Buenos Aires, Argentina, 2 Banco de Tejidos, Hospital de Quemados de la Ciudad de Buenos Aires, Buenos Aires, Argentina and 3 CEBBAD, Universidad Maimónides, Buenos Aires, Argentina The bio-engineered composite dermo-epidemal skin could be a useful tool to treat deep and extensive skin injuries. We previously demonstrated that the presence of human Dermal Papilla Cells (DPC) in a composite skin with Hair Follicle Stem Cells (HFSC) using acellular porcine dermis (APD) as scaffold, contributed to a more arranged stratified-epidermis with more precursor cells and a better graft-take in nude mice. In the present work, we compared the effect of DPC and human dermal fibroblasts (DF) as dermal component, on tissue architecture and graft take of a permanent composite skin with HFSC in immunocompetent mice. For that purpose we grafted mice with APD alone, composite skin with HFSC and DF or HFSC and DPC. In every case, ulcerative lesions with reepithelization- attempts from the edges were observed fourteen days after grafting the animals. Histological analysis revealed that in composite skin cases epidermolysis of graft-epidermis associated to a rejection process was observed. Twenty one days after grafting, composite skin containing DF showed an intensive inflammatory reaction below the epidermis with epidermolysis and a macroscopic evidence of remaining ulcerative lesion with an incomplete re-epithelization from the edges. On the other hand, composite skin containing DPC showed an extensively remodeled APD with almost complete tissue integration, total epidermis sealing of lesion and a macroscopic absence of ulcerative lesions. When xenogeneic cellular components are present in composite skin, the evidence indicates that the epidermis was rejected by the mice immune system. Nevertheless the composite skin epidermis allows transitional lesion coverage. Interestingly, only the presence of DPC favored the scaffold integration and a complete lesion-re-epithelization from the edges.