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Study of the Effects of Sodium Arsenite Exposure in Rat Kidney by Synchrotron Microscopic X-Ray Fluorescence Analysis.
PEREZ, RD; RUBIO, M; PEREZ, CA; EYNARD, ALDO R; BONGIOVANNI, GUILLERMINA
Conferencia; EXRS 2006 (European Conference on X ray Spectrometry; 2006
LNHB-CEA LIST & ENS Lion (www.nucleide.org/exrs2006)
Arsenic is a naturally occurring element widely present in the environment. Inorganic arsenic exposure in humans is causally associated with development of malignancies in various tissues, including skin, liver, urinary bladder, lung and prostate. Vast areas of Argentina have high exposures of human and animals to toxic levels of inorganic arsenic from naturally contaminated drinking water, particularly the eastern region of the province of Córdoba. The problem was imposing in this area due to its remarkable importance for the agricultural development of the rural population. The disease ascribed to arsenic contamination in Argentina was called chronic endemic regional hydroarsenism. Although arsenic is a recognized human carcinogen, the mechanism by which arsenic induces cancer is unknown, in large part due to the lack of an appropriate animal model. The kidney is exposed to high concentrations of arsenic compounds as it filters these compounds into the urine. There are strong evidence showing that mammals fed inorganic arsenic chronically accumulate arsenic in the kidney. Since the effect of arsenic in kidney may be due to its longer retention in the tissue, information on the spatial distribution of arsenic after exposure to inorganic arsenic are of critical importance. Moreover, since arsenic compounds induce redox changes, information on the spatial distribution of other elements are also very important. Micro x-ray fluorescence using synchrotron radiation (mSR-XRF) is a multielementar analytical technique with spatial resolution. The results obtained by this technique indicate that chronic intake of arsenical water induce As and Cu accumulation in kidney of rats. The scanning experimental results showed that the distribution maps of Cl, K, Cu, Zn and As changes with the increase of arsenic dose. At the beginning of the arsenic exposure, the accumulation of As was preferably in the marrow, but then it gradually pass to the renal cortex.