BONGIOVANNI guillermina Azucena
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Synchrotron radiation XRF microprobe analysis applied to the study of As and Cu renal cortex accumulation after chronic arsenic intake
PEREZ, CARLOS A; RUBATTO BIRRI, PAOLO N; PEREZ, ROBERTO D; CREMONEZZI, DAVID; RUBIO, MARCELO; BONGIOVANNI, GUILLERMINA A.
Congreso; 20th International Congress on X-ray Optics and Microanalysis (ICXOM20); 2009
The X-ray Fluorescence (XRF) analysis is a well-established method for quantitative multi-elemental bulk analysis . When primary radiation is focused to micrometer dimensions by suitable optics, a nondestructive and non-invasive method of analysis is obtained. The use of a synchrotron radiation source allows constructing effective x-ray microprobes for study trace elements in small (nanogram) samples or their distributions with high spatial resolution. Since its operation, the XRF fluorescence beamline of the LNLS has offered to the user community several hard x-ray microprobes configurations to develop microscopic x-ray fluorescence analysis. Particularly interesting is the application of XRF microprobe analysis to study biological systems . It is known that arsenic (As) is one of the most abundant hazards in the environment and it is a human carcinogen. Related you excretory function, the kidneys in humans, animals models or naturally exposed fauna are target organs for As accumulation and deleterious effects. Previous studies carried out by x-ray fluorescence analysis using synchrotron radiation (SR- XRF) showed a high concentration of As in renal cortex from chronically exposed rats, suggesting that it is a suitable model for studies on renal As accumulation . This accumulation was accompanied by an important increase in the copper (Cu) accumulation. The present study focused on the localization of these elements along renal cortex and their correlation with physiological and histological As-related renal effects. This study supports the hypothesis that tubular accumulation of As-Cu may have some bearing on the arsenicassociated nephrotoxicological process.