BONGIOVANNI guillermina Azucena
congresos y reuniones científicas
Multielemental analysis and arsenic determination in renal cortex of rats by SR-µTXRF
PEREZ, RD; RUBATTO BIRRI, PN; PEREZ, CA; RUBIO, M; BONGIOVANNI, GUILLERMINA
Campinas, Sao Paulo, Brasil
Otro; 17 Reunión Anual de Usuarios de Síncrotron; 2007
Laboratorio Nacional de Luz Síncrotron
Arsenic is an environmental toxicant and a human carcinogen. The kidney, a known target organ of arsenic toxicity, accumulates this element. In previous in vitro studies, has been reported that As Chronic exposure to lower levels of arsenic can cause adverse health effects, including vascular disease, peripheral neuropathy, exacerbation of the complications of diabetes, cardiac arrhythmias, liver and kidney toxicity, anemia and leukopenia. Although the mechanisms not has been fully determined, arsenic exerts its toxicity by inactivating up to 200 enzymes, possibly an adverse affect on DNA repair, methylation of DNA, and increased free radical formation and activation of the proto-oncogene c-myc. In order to determinate who is the cellular fraction target to arsenic incorporation, the multielemental analysis was performed in supernatant and total membranes fractions from rat brain, pancreas, kidney and liver by SR-TXRF. For that, those organs from rats drinking arsenical water (50 ppm ) during 0, 30 or 60 days were homogenized and centrifugated at 100,000 x g. We observed that arsenic fluorescence intensity was higher in kidney and liver than in brain or pancreas. Furthermore, we found arsenic increased in soluble cellular fractions. Additionally, other elements as P, Cl, Ca, Fe, Cu and Zn showed also an altered proportion. The results suggest specific cellular components-arsenic interactions and that the proportion of other elements is also altered by the chronic intake of arsenic.