INVESTIGADORES
BONGIOVANNI guillermina Azucena
artículos
Título:
Study of the effects of chronic arsenic poisoning on rat kidney by means of synchrotron microscopic X-ray fluorescence analysis
Autor/es:
PEREZ, RD; RUBIO, M; PEREZ, CA; EYNARD, ALDO R; BONGIOVANNI, GUILLERMINA
Revista:
X-RAY SPECTROMETRY
Editorial:
Wiley InterScience
Referencias:
Año: 2006 vol. 35 p. 352 - 358
ISSN:
0049-8246
Resumen:
Synchrotron microscopic x-ray fluorescence analysis (m-SRXRF) was used for determining the twodimensional
distribution of Cl, K, Fe, Cu, Zn, As and Br concentrations in rat kidney slices. The animals
received drinking water containing 100 ppm of sodium arsenite ad libitum for 30 and 60 days. Lyophilized
kidneys sectioned from normal and treated rats were scannedwith a collimated white synchrotron spectrum
(300?~ 300 Êm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure
was corroborated, and the spatial distributions of these elements were studied in detail. While copper was
restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A
correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused
by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions
under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were
no significant changes in iron and bromine but the patterns of their spatial distributions were clearly
identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to
detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and
suggest interesting hypotheses. Copyright 2006 John Wiley & Sons, Ltd.ad libitum for 30 and 60 days. Lyophilized
kidneys sectioned from normal and treated rats were scannedwith a collimated white synchrotron spectrum
(300?~ 300 Êm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure
was corroborated, and the spatial distributions of these elements were studied in detail. While copper was
restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A
correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused
by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions
under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were
no significant changes in iron and bromine but the patterns of their spatial distributions were clearly
identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to
detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and
suggest interesting hypotheses. Copyright 2006 John Wiley & Sons, Ltd.?~ 300 m-SRXRF) was used for determining the twodimensional
distribution of Cl, K, Fe, Cu, Zn, As and Br concentrations in rat kidney slices. The animals
received drinking water containing 100 ppm of sodium arsenite ad libitum for 30 and 60 days. Lyophilized
kidneys sectioned from normal and treated rats were scannedwith a collimated white synchrotron spectrum
(300?~ 300 Êm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure
was corroborated, and the spatial distributions of these elements were studied in detail. While copper was
restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A
correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused
by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions
under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were
no significant changes in iron and bromine but the patterns of their spatial distributions were clearly
identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to
detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and
suggest interesting hypotheses. Copyright 2006 John Wiley & Sons, Ltd.ad libitum for 30 and 60 days. Lyophilized
kidneys sectioned from normal and treated rats were scannedwith a collimated white synchrotron spectrum
(300?~ 300 Êm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure
was corroborated, and the spatial distributions of these elements were studied in detail. While copper was
restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A
correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused
by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions
under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were
no significant changes in iron and bromine but the patterns of their spatial distributions were clearly
identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to
detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and
suggest interesting hypotheses. Copyright 2006 John Wiley & Sons, Ltd.?~ 300 Êm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure
was corroborated, and the spatial distributions of these elements were studied in detail. While copper was
restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A
correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused
by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions
under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were
no significant changes in iron and bromine but the patterns of their spatial distributions were clearly
identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to
detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and
suggest interesting hypotheses. Copyright 2006 John Wiley & Sons, Ltd.m-SRXRFis a very well-positioned and precise technique to
detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and
suggest interesting hypotheses. Copyright 2006 John Wiley & Sons, Ltd. 2006 John Wiley & Sons, Ltd.