BONGIOVANNI guillermina Azucena
MCF 7 cells apoptosis induced by Larrea divaricata Cav. extracts.
BONGIOVANNI, GUILLERMINA; CANTERO, JJ; EYNARD, ALDO R; GOLENIOWSKI, ME
Journal of Experimental Therapeutics and Oncology
Old City Publishing, Inc
Año: 2008 vol. 7 p. 1 - 7
The methanolic and methane dichloride extracts of aerial parts of Larrea divaricata Cav. (Jarilla) plants exhibited a pronounced cytotoxic effect by arresting cell viability at a level of 35 % against the MCF 7 cell line, a human breast adenocarcinoma, while only a weak cytotoxic effect was observed for the aqueous extract. Apoptosis was detected by flow cytometry, using annexin V and by cytological detection with fluorescein diacetate / propidium iodide staining. The annexin V, as well as the uptake of fluorescein diacetate/propidium iodide staining, revealed that methanolic and methane dichloride extracts of L. divaricata treatment of cells resulted in a rapid plasma membrane perturbation and triggered cellular death (>70 %). Methanolic extracts were submitted to further treatment by bioassay-guided purification, involving fractionation and chromatography. 0.1 % (w/w) nordihydroguaiaretic acid (NDGA) was found, which displayed weak citotoxic activity.