INVESTIGADORES
BONGIOVANNI Guillermina Azucena
artículos
Título:
Study of the effects of chronic arsenic poisoning on rat kidney by means of synchrotron microscopic X-ray fluorescence analysis
Autor/es:
PEREZ, RD; RUBIO, M; PEREZ, CA; EYNARD, ALDO R; BONGIOVANNI, GUILLERMINA
Revista:
X-RAY SPECTROMETRY
Editorial:
Wiley InterScience
Referencias:
Año: 2006 vol. 35 p. 352 - 358
ISSN:
0049-8246
Resumen:
Synchrotron microscopic x-ray fluorescence analysis (m-SRXRF) was used for determining the twodimensional distribution of Cl, K, Fe, Cu, Zn, As and Br concentrations in rat kidney slices. The animals received drinking water containing 100 ppm of sodium arsenite ad libitum for 30 and 60 days. Lyophilized kidneys sectioned from normal and treated rats were scannedwith a collimated white synchrotron spectrum (300?~ 300 ƒÊm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure was corroborated, and the spatial distributions of these elements were studied in detail. While copper was restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were no significant changes in iron and bromine but the patterns of their spatial distributions were clearly identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and suggest interesting hypotheses. Copyright  2006 John Wiley & Sons, Ltd.ad libitum for 30 and 60 days. Lyophilized kidneys sectioned from normal and treated rats were scannedwith a collimated white synchrotron spectrum (300?~ 300 ƒÊm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure was corroborated, and the spatial distributions of these elements were studied in detail. While copper was restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were no significant changes in iron and bromine but the patterns of their spatial distributions were clearly identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and suggest interesting hypotheses. Copyright  2006 John Wiley & Sons, Ltd.?~ 300 m-SRXRF) was used for determining the twodimensional distribution of Cl, K, Fe, Cu, Zn, As and Br concentrations in rat kidney slices. The animals received drinking water containing 100 ppm of sodium arsenite ad libitum for 30 and 60 days. Lyophilized kidneys sectioned from normal and treated rats were scannedwith a collimated white synchrotron spectrum (300?~ 300 ƒÊm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure was corroborated, and the spatial distributions of these elements were studied in detail. While copper was restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were no significant changes in iron and bromine but the patterns of their spatial distributions were clearly identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and suggest interesting hypotheses. Copyright  2006 John Wiley & Sons, Ltd.ad libitum for 30 and 60 days. Lyophilized kidneys sectioned from normal and treated rats were scannedwith a collimated white synchrotron spectrum (300?~ 300 ƒÊm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure was corroborated, and the spatial distributions of these elements were studied in detail. While copper was restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were no significant changes in iron and bromine but the patterns of their spatial distributions were clearly identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and suggest interesting hypotheses. Copyright  2006 John Wiley & Sons, Ltd.?~ 300 ƒÊm). The accumulation of arsenic and copper in the rat kidney induced by arsenic exposure was corroborated, and the spatial distributions of these elements were studied in detail. While copper was restricted to the renal cortex, arsenic showed changes in its spatial distribution suggesting nephrotoxicity.A correlation between the spatial distributions of zinc and arsenic was observed, which appears to be caused by the antioxidant properties of zinc. Chlorine and potassium also changed their spatial distributions under arsenic exposure, showing a correlation probably to maintain electrical neutrality. There were no significant changes in iron and bromine but the patterns of their spatial distributions were clearly identified. The obtained results show that m-SRXRFis a very well-positioned and precise technique to detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and suggest interesting hypotheses. Copyright  2006 John Wiley & Sons, Ltd.m-SRXRFis a very well-positioned and precise technique to detect the spatial distributions of elements in mammalian tissues in healthy and diseased conditions and suggest interesting hypotheses. Copyright  2006 John Wiley & Sons, Ltd. 2006 John Wiley & Sons, Ltd.