Seasonal seminal quality variations and vitrification prospects in black flounder Paralichthys orbignyanus

ZALAZAR, L.; NICOLLI, ANABELLA R.; LOPEZ A; RADONIC M; CESARI A

THERIOGENOLOGY

ELSEVIER SCIENCE INC

Año: 2024 vol. 229 p. 30 - 40

0093-691X

Long-term preservation of gametes has been identified as a tool to improve broodstock management and increasethe number of juveniles produced by artificial fertilization. Paralichthys orbignyanus is an important commercialand recreational species distributed in marine and estuarine waters from Rio de Janeiro (Brazil) to the San MatíasGulf (Argentina). This work focused on studying the seminal quality of tank-reared P. orbignyanus, demonstratingthat males are fluent year-round, with the highest yields at the early reproductive season. Fresh sperm exhibitedgood forward swimming, and samples could be refrigerated up to 48 h while retaining their motility afteractivation. The optimal conditions for P. orbignyanus sperm motility activation were established as 950 mOsmol/Kg and pH values between 7 and 7.9. Additionally, a well-defined protocol for semen vitrification was developedto assess the cryotolerance of this species’ sperm. We successfully produced high-quality sperm samples, usingtwo vitrification formulations containing trehalose and both z-1000 and x-1000 polymers, that can be used in anear-future in vitro embryo production program.