INVESTIGADORES
CHECA Susana Karina
congresos y reuniones científicas
Título:
Operator discrimination between paralog MerR regulators in Salmonella
Autor/es:
SONCINI FC; CHECA SK; IBÁÑEZ MM; ZAFRA M; HUMBERT MV; CERMINATI S; PÉREZ AUDERO ME.
Lugar:
Tucson
Reunión:
Simposio; 7th International Biometals Symposium; 2010
Institución organizadora:
University of Arizona
Resumen:
All cells possees a battery of highly specific regulaory proteins that control metal ions homeostasis by modulating the balance of intake/efflux of a particular metal ion. This control is fundamental to guarantee the adquisition of the required amount of essential metals and at the same time to avoid toxicity caused either by their overload or by the presence of toxic metals. In Salmonella, two highly homologous transcription factors CueR and GolS are responsible for sensing and resistance to Cu and Au, respectively. They share similarities not only in their highly homologous, phylogenetic analysis shows that these two proteins cluster separately regulators are extended to their target transcription binding sites. Indeed, these regulators can distinguish operators by recognizing specific nucleotide bases at the promoter region of their target genes. Promoter architecture plus the environmentally-controlled regulator´s cytoplasmic availability warrant in vivo regulator-operator selectivity, and the proper response to metal injury. The distinction in target transcription binding sites is widely extended among GolS-and CueR-like factors present in Gram negative species. This approach allows the identification of genes under the control o fthese transcription factors and the prediction of transcription modules among these goups of highly homologous MerR monovalent metal ion regulators in yet unchracterized bacterial species.