Phosphorylation of an envelope-associated Hsp70 homolog in amyloplasts isolated from cultured cells of sycamore (Acer pseudoplatanus L.)

SUSANA KARINA CHECA; VIALE, A. M.

CELLULAR AND MOLECULAR BIOLOGY

C.M.B. Association

Lugar: Francia; Año: 1998 vol. 44 p. 891 - 897

0145-5680

Calification SCImago-Q2, IF (2013) 0.691. The presence of Hsp70 and Hsp60 molecular chaperones in amyloplasts isolated from cultured sycamore cells was analyzed by immunoblotting. Hsp70 homologs were located in both amyloplast envelope and stromal fractions, but no Hsp60 homologs were detected in any of the different suborganellar fractions. Incubation of whole amyloplasts or their envelope fraction with Mg2+ gamma-32P-ATP resulted in a rapid phosphorylation of the envelope-associated Hsp70 homolog, which constitutes a major target of phosphorylation in these plastids.