INVESTIGADORES
ALVARENGA adriana elizabet
congresos y reuniones científicas
Título:
Caracterizacion y Comparacion mediante Estudios Fisiologicos, Bioquimicos y Moleculares de dos cepas productoras de Xilanasas Frio Activas
Autor/es:
ALVARENGA, A.E.; ABATE, C.M.
Lugar:
Tucuman. Argentina.
Reunión:
Jornada; XXV JORNADAS CIENTIFICAS. Asociacion de Biologia de Tucuman; 2008
Institución organizadora:
Asociacion de Biologia de Tucuman
Resumen:
At present, marine microorganisms represent a source of biotechnological and industrial interest due their capability to elaborate cool-active biocatalizers such as xylanases. The aims of the present work were: 1) Physiological characterization of the strains using API 20NE test and growth curves at 4 °C and 20 °C. 2) Biochemical studies of xylanolitic activity. 3) Molecular study by 16S DNAr-amplification restriction analyses, intergenic sequences (ITS) and protein profile of the strains. Starting from 70 isolations from different coastal areas of the Beagle Channel (Ushuaia, Argentina), two strains called P2  and P8 were selected by qualitative techniques because they presented xylanase activity at 8¨¬C and 20¨¬C. The P8 strain presented highest specific activity at 25¨¬C. Filogenetic relationships for the isolations established by 16S RNA gene amplification allowed us to locate them within the gamma- Proteobacteria subclass, as belonging to the  Pseudoalteromonas genus. The differences found in proteic and ITS profiles provide evidence that P2 and P8 isolations correspond to different strains. Marine bacteria identification isolated from subantarctic ecosystems contributes to increase knowledge of marine microorganisms diversity in the Beagle Channel.