Secretomic analysis of cheap enzymatic cocktails of Aspergillus niger LBM 134 grown on cassava bagasse and sugarcane bagasse

DÍAZ, G. V.; CONIGLIO, R.O.; ALVARENGA A. E.; ZAPATA, P.D.; VILLALBA, L.L.; FONSECA M.I.

MYCOLOGIA.

ALLEN PRESS INC

Lugar: Lawrence; Año: 2020

0027-5514

Currently, agroindustrial wastes are little used for generating added-value products; hence, the use of them to produce enzymatic cocktails for the conversion of lignocellulosic biomass to fermentable sugars is a very interesting alternative in the 2G bioethanol process. The Ascomycota fungus Aspergillus niger LBM 134 produces hydrolytic enzymes in large proportions. In this work, A. niger LBM 134 was grown on sugarcane and cassava bagasses under optimized conditions. To identify the extracellular enzymes involved in the degradation of these agroindustrial wastes, the secretomes of the culture supernatants of the fungus were analyzed and validated by biochemical assays of the enzymatic activities. A. niger LBM 134 secreted more quantities of xylanases and accessory hemicellulases when it grew on sugarcane bagasse, while more cellulases, amylases and pectinases were secreted when it grew on cassava bagasse. These findings can be considered as two promising enzyme cocktails for the hydrolysis of lignocellulose carbohydrate polymers to fermentable sugars. These bioinformatic analysis were functional validates through enzymatic biochemical assays that confirm the biotechnological potential of A. niger LBM 134 for the bioconversion of hemicellulosic substrates such as sugarcane and cassava bagasse.