NUSBLAT Alejandro David
congresos y reuniones científicas
Heterologous expression of glycosylphosphatidyl (GPI)-anchored vaccine candidates of bovine pathogenic protozoa in Tetrahymena thermophila
MONTES, GUADALUPE; EUGENIA ELGUERO; M. L. TOMAZIC; RODRIGUEZ ANABEL; B.C. NUDEL; FLORIN-CHRISTENSEN M; LEONHARD SCHNITTGER; A. D. NUSBLAT
Conferencia; VACCINES RESEARCH AND DEVELOPMENT; 2016
Tetrahymenathermophila is a free-living non-pathogenic ciliatethat is suitable for recombinantexpression of glycosylphosphatidylinositol (GPI)-anchored antigens. Several glycosylphosphatidylinositol (GPI) anchors proteins have been expressed successfully in the surface of this ciliate. Some examples are the circumsporozoite protein (CSP) of Plasmodium falciparum and the immobilization antigen of Ichthyophthirius multifiliis, which are used as vaccine candidates for malaria and white dot disease respectively.Babesia bovis is a tick-transmitted hemoprotozooan that causes infection diseases invertebrate hosts. It is responsible for the mortality of cattles, leading to significant economic losses. Cryptosporidium parvum is an apicomplexanprotozoa responsible for high morbidity and mortality of calves raised in dairyfarm settings. Up to date, no effective recombinantvaccines are available. The GPI proteins MSA2c of B. bovis and GP60 of C.parvum represent prominent vaccine candidatesthat were chosento be expressed in T. thermophila for the preparation of subunitvaccines.