Pleurotus pulmonarius MUSHROOM AS NOVEL RESOURCES OF PREBIOTICS: EFFECT ON THE GROWTH AND RESISTANCE TO PASSAGE THROUGH THE GASTROINTESTINAL TRACT OF LACTIC ACID BACTERIA

GÓMEZ, JORGE N; LEDESMA, ANA E; TARANTO, MARIA P; BUSTOS, ANA Y

San Miguel de Tucumán

Simposio; VI International Symposium on Lactic Acid Bacteria - I Argentinean-Japanese Lactic Acid Bacteria "Tohoku Forum for Creativity" Meeting; 2024

Centro de Referencia para Lactobacilos

Prebiotics improve host health by promoting the growth and metabolic activity of beneficial strains such as probiotic lactic acid bacteria (LAB). Due to their numerous benefits, the search for and characterization of new prebiotics is an area of growing interest. In this sense, the Pleurotus (P.) pulmonarius mushroom is rich in non-digestible carbohydrates that could act as prebiotics and favor the growth and activity of beneficial bacteria. The objective of this work was to extract potential prebiotic oligosaccharides from P. pulmonarius and evaluate their ability to modulate the growth and resistance to simulated gastrointestinal tract (SGIT) conditions of probiotic LAB. For this, the relative growth rate (RGR), prebiotic activity (PA), prebiotic index (PI) and survival through SGIT of selected LAB strains in the presence of mushroom oligosaccharides was evaluated. The strains were inoculated in MRS broth supplemented with fungal carbohydrates as the sole carbon source or with glucose or commercial FOS as controls. For Escherichia (E.) coli, the LB medium was used under the same conditions. Our results showed that fungal carbohydrates exert a positive effect on the growth of certain LAB in relation to glucose and FOS as Levilactobacillus (L.) brevis ATCC 14869, Lactiplantibacillus (L.) plantarum CB11 and Lentilactobacillus (L.) parabuchneri CB12 presented RGR and PA > 1. On the other hand, Bifidobacterium longum DSM 20219, and Limosilactobacillus reuteri ATCC 23272 did not grow in the presence of fungal carbohydrate or FOS. A PI of 0.47-0.55 was obtained, indicating that the extract was selectively utilized by beneficial microorganisms in relation to glucose and its use was limited in enteric bacteria. After gastric passage, no significant difference was observed compared to the initial count. After incubation in intestinal juice, the colony-forming capacity of L. brevis and L. plantarum CB11 decreased approximately 0.5 log units, when glucose was used as a carbon source, while in the presence of fungal oligosaccharides, viability was maintained at initial levels of 8 log CFU/mL. Our results indicate that P. pulmonarius can stimulate the growth and resistance to the SGIT step of certain probiotic strains, which would allow its use in the design of symbiotic prototypes.