10. Small angle scattering techniques for biomolecular structure and dynamics

A. M. MEBERT; M. E. VILLANUEVA; G. I. TOVAR; C. J. PEREZ; G. J. COPELLO

Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics

Elseiver

Lugar: Amsterdam; Año: 2022; p. 271 - 307

Small-angle scattering (SAS) techniques provide information regarding molecular, macromolecular, and supramolecular distances; interactions; shapes; composition; and so on. These techniques involve small-angle X-ray scattering, small-angle neutron scattering, and small-angle light scattering when using X-rays, neutrons, or visible light as probes, respectively. At a spatial range between 1 and 100 nm, the type of information that SAS techniques bring roughly overlaps with other techniques. The closest relatives are dynamic and static light scattering and microscopy techniques, but neither can provide information on biomolecular dynamics in the way that SAS techniques can. SAS techniques have been hyphenated to many other characterization techniques to obtain relevant information under nonequilibrium conditions. Aided by powerful modeling, these techniques have gained an indisputable place in biomolecule analysis and are becoming more widespread. This chapter aims to present the state-of-the-art SAS techniques for the inexperienced and SAS enthusiast users.