VIP conditions human endometrial receptivity by privileging endoplasmic reticulum Stress through ATF6α pathway

E.SOCZEWSKI; S.GORI; D. PAPARINI ; E. GRASSO ; L. FERNÁNDEZ ; L. GALLINO ; A. SCHAFIR ; M.IRIGOYEN; T.F.LOBO; G.SALAMONE; R.MATTAR; S.DAHER; C.PÉREZ LEIRÓS; R. RAMHORST

MOLECULAR AND CELLULAR ENDOCRINOLOGY.

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2020

0303-7207

Endometrial stromal cells undergo endoplasmic reticulum (ER) stress and unfolded protein response (UPR) during the decidualization linked with the inflammation and angiogénesis processes. Considering VIP (vasoactive intestinal peptide) induces the decidualization program, we studied whether modulates the ER/UPR pathways to condition both processes for embryo implantation. When Human Endometrial Stromal Cell line (HESC) were decidualized by VIP we observed an increased expression of ATF6α, an ER stress-sensor, and UPR markers, asso ciated with an increase in IL-1β production. Moreover, AEBSF (ATF6α -inhibitor pathway) prevented this effect and decreased the expansion index in the in vitro model of implantation. VIP decidualized cells also favor angio genesis accompanied by a strong downregulation in thrombospondin-1. Finally, ATF6α, VIP and VPAC2-receptor expression were reduced in endometrial biopsies from women with recurrent implantation failures in comparison with fertile. In conclusion, VIP privileged ATF6α-pathway associated with a sterile inflammatory response and angiogenesis that might condition endometrial receptivity.