BECAS
BRITEZ NEIRA Diego Javier
congresos y reuniones científicas
Título:
Effect of Lapatinib and All-Trans Retinoic Acid (ATRA) combined treatment on mammary cancer stem cells derived from HER2 negative cell lines
Autor/es:
BECHIS, ANDRES; ARIZA BAREÑO, LIZETH AIXA; BRITEZ NEIRA, DIEGO JAVIER; URTREGER, ALEJANDRO JORGE
Lugar:
CABA
Reunión:
Simposio; BUENOS AIRES BREAST CANCER SYMPOSIUM BA-BCS 2021; 2021
Resumen:
Cancer stem cells (CSC) are resistant to chemo and radiotherapies. To validate CSC as therapeutic targets in breast cancer, we analyzed the effect of Lapatinib (Lp, HER2 inhibitor), ATRA or the combined treatments, on growth, cell cycle distribution and metastatic capacity of primary mammospheres (CSC enriched cultures) from HER2 negative cell lines (4T1, MCF-7 and T47D). We determined by WB that HER2 is overexpressed only in CSC subpopulation of all cell lines analyzed. Primary mammospheres were treated for 96 h with Lp1µM for 4T1; 5µM for MCF-7, 2µM for T47D cells combined or not with ATRA 1µM. ATRA treatment alone or combined with Lp only significantly reduced 4T1 mammospheres diameters (p
