Chlorpyrifos induces citotoxicity, B-esterase inhibitoion and oxidative stress in endothelial cells

BRITEZ NEIRA, DIEGO JAVIER; ROCCO, RODRIGO; GAZZANIGA SILVINA; COCHÓN ADRIANA

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

Chlorpyrifos (CPF) is an organophosphate insecticide widely used in Argentina and throughout the world. In animals, exposure to CPF produces inhibition of B-esterases (acetylcholinesterase, AChE, and carboxylesterases, CES) and multiple toxic effects. However, little is known about the effect of CPF on endothelium. The aim of this work was to explore in vitro effects of CPF on endothelial cholinesterase and non-cholinesterase targets. Mouse endothelial cell cultures (H5V) were treated with CPF for 24 h. The biochem- ical parameters analyzed were AChE, CES, antioxidant enzymes (catalase, CAT and superoxide dismutase, SOD) and the levels of reduced (GSH) and oxidized (GSSG) glutathione. Enzyme activities were measured spectrophotometrically and GSH and GSSG levels were determined by the recycling method using GSH reductase. ROS production was determined by flow cytometry with the fluorescent probe DCF-DA and the morphological analysis was performed with Image J using phase-contrast images. When analyzing cell morphology, a decrease in cell area was observed at CPF concentrations > 50 μM (p