Biogenesis of extracellular vesicles released by the parasite Trichomonas vaginalis

SALAS, NEHUEN; ANTONIO, PEREIRA-NEVES; VERONICA, COCERES; NATALIA, DE MIGUEL

Congreso; XXXI Anual Meeting of Sociedad Argentina de Protozoologia; 2019

Trichomonas vaginalis is a parasite that causes trichomoniasis, the most common non-viral sexually disease worldwide. Given it is an extracellular parasite, adhesion to host cells is one of the key processes for the development of infection. In recent years, various factors that influence this process have been identified, including extracellular vesicles (EVs). Previously in our laboratory we demonstrated that both, exosomes (vesicles with a size range of 40-100 nm) and microve- sicles (vesicles with a size range of 100 - 1000 nm), are involved in the process of interaction of T. vaginalis with host cells.Recently, it has been demonstrated that members of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery are involved regulation of EVs biogenesis in mammalian cells. In this study, the role of VPS32, a member of the ESCRTIII complex subunit and the key effector of cleavage during membrane scission, in EVs formation in T. vaginalis was examined.