Endocannabinoid system caracterization in decidual tissues from 15-day pregnant mice

MARVALDI, CAROLINA; SCHANDER, JULIETA AYLEN; HERRERO LO GUIDICE, FELISA MARIA; AISEMBERG, JULIETA; FRANCHI, ANA MARIA; WOLFSON, MANUEL LUIS

Mar del Plata

Congreso; Reunion conjunta SAIC SAI SAFIS 2018; 2018

SAIC

Endocannabinoids, like anandamide (AEA), are theendogenous ligand for the cannabinoid receptors CB1 and CB2. Similarly to prostaglandins,endocannabinoids are implicated in different aspects of reproduction, such asmaintenance of pregnancy and parturition. On the other hand, we known that indeciduas from 7-day of pregnant mice the lipopolysaccharide (LPS) increased thelevel of prostaglandin F production and the protein level of cyclooxygenase(COX)-2. The aim of the present studywas to investigate the presence and possible modulation of the ECS in deciduatissue in LPS-induced preterm labor model.We demonstrated the presence of endocannabinoidsystem in deciduas from 15-day of pregnant Balb/c mice, comprising the enzymethat synthesizes AEA, (N-acylphosphatidylethanolamine-specificphospholipase D, NAPE-PLD,), the enzyme that hydrolase AEA (fatty acid amidehydrolase, FAAH), and the receptors CB1 and CB2. Weobserved in deciduas from a model of preterm labor LPS-induced in day 15 ofpregnancy an increase in the COX-2 protein level vs deciduas from control15-day pregnant mice (p<0.05).Furthermore,we utilized an in vitro model to evaluate COX-2 protein level. Briefly, deciduafrom 15-day pregnant mice were obtained and cultured in presence or not of LPSfor 6 hours. The treatment with LPS increased the protein levels of COX-2 in15-day of pregnant decidua explants (p<0.05).  In summary, we showedthat the ECs is present in the decidual tissue and we proposed an in vitroapproach to study the changes induced by LPS and its possible modulation forthe ECS.