COMPARATIVE ANALYSIS OF THE BIOLOGICAL ACTIVITY OF DIFFERENT G-CSF ANALOGUES ON MOBILIZATION AND DIFFERENTIATION OF MURINE PLURIPOTENT BONE MARROW CELLS.

MATERAZZI, LOURDES; MARVALDI, CAROLINA; ACEBEDO, MACARENA; GIAMBALVO GÓMEZ, DILEYVIC; FERRAIOLO, PAULA; MAZZEY, J; DIEZ, ROBERTO; LOMBARDI, MARIA GABRIELA

Mar del Plata

Congreso; Reunion anual de sociedades de biociencias; 2022

Sociedad Argentina de Investigación Clinica

Granulocyte colony-stimulation factor (G-CSF) is a cytokine that promotesgrowth and maturation of neutrophil progenitor cells by interacting with aspecific cell receptor (G-CSFR). It can also mobilize progenitor cells from thebone marrow into peripheral blood that can be used for hematopoieticreconstitution. In this work, we analysed two recombinant proteins produced bybiotechnology based on the G-CSF gene that are used in clinical medicine:lenograstim (L; eukaryotic glycosylation) and filgrastim (F; non-glycosylated).Comparisons between these proteins are limited, but it is generally acceptedthat they function as full agonists of G-CSFR and have equivalent efficacy intheir clinical capabilities. However, conflicting reports have emerged regardingthe effects obtained with both forms of G-CSF. Here, we use a cytopenia modelinduced by a single dose of cyclophosphamide (300 μg/g), in 12-week-old CD-1mice. After 4 days, the G-CSF analogues were administered or not during 4days (daily dose:300 μg/g). Then, a blood smear was performed and bloodsample were collected. Labelling of peripheral blood mononuclear cells with anantibody against CD117 (c-Kit receptor) showed that F and L are able tosignificantly increase the quantity of circulating haematopoietic precursors,compared to control(*p