BECAS
ANSELMI RELATS Juan Manuel
congresos y reuniones científicas
Título:
MECHANISM OF 2´-NITROFLAVONE AND SAFINGOL ANTITUMOR SYNERGISTIC COMBINATION IN MAMMARY CANCER CELLS
Autor/es:
JUAN MANUEL ANSELMI RELATS; LEONOR ROGUIN; MARIEL MARDER; JULIETA MARINO; VIVIANA BLANK
Reunión:
Congreso; Reunión Anual de Sociedades de Biociencias; 2022
Resumen:
Sphingosine kinase 1 (SphK1), a lipid kinase overexpressed in some mammary tumor cells, regulates the balance between proapoptotic ceramides and prosurvival sphingosine-1-phosphate. Furthermore, SphK1 inhibitors prevent catabolism of ceramides, contributing to tumor cell death. It has been reported that certain flavonoids exert antitumor activity through an increment in ceramide levels. Previously, we demonstrated that the synthetic flavonoid 2´-nitroflavone (2NF) and the SphK1 inhibitor safingol synergistically inhibited cell proliferation and induced apoptosis in LM3 murine mammary tumor cells. In this work, we examined the modulation of JNK and ERK 1/2 MAPK transduction pathways. Results showed that after 1h of incubation with both compounds, the levels of p-JNK and p-ERK1/2 MAPKs were significantly increased (2,3 ± 0,3 fold, p