Vav2 and Vav3 impact immunological features and cellular characteristics in melanoma

MACARENA MAMBERTO; NAHUEL CESATTI LALUCE; AYLEN AVILA; LUCIANO ANSELMINO; MAURICIO MENACHO MÁRQUEZ

Otro; Immuno-Morocco course 2023: Tumor immune microenvironment and immunotherapies; 2023

Melanoma is the most dangerous form of skin cancer, associated with an increasing incidence in the population. Vav proteins, GEFs for Rho GTPase, surprisingly, play opposing roles in mouse melanoma cell line B16-F0, as we previously found.In our study, we explored how Vav2 and Vav3 regulate immunological aspects in melanoma. Microarray analysis revealed that Vav2 expression leads to downregulation in the antigen presentation folding pathway and immunoregulatory interactions. Additionally, Vav3 expression was associated with downregulation in the interferon pathway and the complement cascade.We perform tumor microenvironment studies by subcutaneously injection of the B16-F0 cell lines mentioned above in C57BL/6 mice. We analysed by flow cytometry immune infiltrates in tumor and blood samples. In blood, we observed that mice injected with cells that express Vav3 present more granulocytes and less B lymphocytes in relation with control (p